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KMID : 0624620090420050304
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BMB Reports
2009 Volume.42 No. 5 p.304 ~ p.309
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Effects of nanoparticulate saponin-platinum conjugates on 2,4-dinitrofluorobenzene-induced macrophage inflammatory protein-2 gene expression via reactive oxygen species production in RAW 264.7 cells
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Kim Young-Jin
Kim Dong-Bum
Lee Young-Hee
Lee Young-Hee
Choi Soo-Young
Park Jin-Seu
Lee Seung-Yong
Park Joon-Won
Kwon Hyung-Joo
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Abstract
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Nanoparticulate platinum (II) (nano Pt) is a powerful antioxidant that is widely used to scavenge reactive oxygen species (ROS). The antioxidant activity of nano Pt has gained attention as a potentially useful therapeutic for a variety of diseases including cancer and aging. In the present study, we prepared nanoparticulate saponin-Pt (II) (nano saponin-Pt) conjugates using the ethanol reduction method to enhance the permeability and retention effect of Pt. The nano saponin-Pt conjugates were found to restore the viability of approximately 40% of 2,4-dinitrofluorobenzene (DNFB)-treated RAW 264.7 cells. In addition, we found that nano saponin-Pt conjugates acted as a potent antioxidant that reduced the production of ROS and inhibited activation of the MAP kinase pathway and MIP-2 gene expression in response to DNFB. These results provide insight into the potential usefulness of nano saponin-Pt conjugates as a treatment for contact hypersensitivity.
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KEYWORD
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DNFB, Macrophages, MIP-2, Nano saponin-Pt, ROS
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